Melatonin test
Quick answer: This test measures melatonin, a hormone that helps control the sleep and wake cycle. It is mostly used in specialist care or research for sleep and body-clock problems.
- SampleBlood
- TakesAbout 5 minutes for one blood draw; timed tests can take several hours
- Before the testMelatonin changes through the day and with light, so follow the timing your doctor or the lab gives you.
How the test is done
- 1The test is usually timed for the evening or night
- 2You may be asked to stay in dim light before the samples
- 3Blood, saliva or urine samples are collected at set times
- 4The lab measures melatonin in each sample
What your results can mean
Reading your report
Results depend heavily on the time the sample was taken; discuss them with your doctor.
If the result is outside the usual range
The timing and level of melatonin can point to a shifted body clock, such as delayed sleep phase. Melatonin supplements, light and some medicines can change the results.
Who usually has this test
People with long-lasting problems with sleep timing, usually in specialist sleep clinics or research.
Risks and aftercare
There is no special aftercare, though bruising is possible if blood is drawn.
Names and options
Also called: serum melatonin, sleep hormone test
Listed by the hospital as
- MELATONIN, SERUM
Questions about this test
Do melatonin supplements affect the test?
Yes, melatonin supplements will raise the result, so tell your doctor if you take them. Your doctor will advise you on what to do before the test.
Why is the melatonin test done at night?
Melatonin rises in the evening in dim light and is very low during the day. Testing in the evening shows when your rise begins.
Can a melatonin test diagnose insomnia?
No. Insomnia is diagnosed from your symptoms and sleep history. The test may help only with specific body clock problems.
Related tests
Tamara Hospital & IVF Centre, Rajajinagar
34/3, 10th Cross Road, opp. Vardhaka Sardar Patel School, 1st N Block, 2nd Stage, Rajajinagar, Bengaluru 560010
The hospital is open 24 hours. Call before you come to check the timing for your scan or sample collection.
Get directions ↗IVF Pulse fertility care at Tamara Hospital →
Reception
Waiting area
IVF lab
This page explains each test in general terms so you know what to expect. It is not medical advice: your doctor decides which tests you need and what your results mean. Reference ranges differ between laboratories. Updated 4 November 2026.