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Blood count and clotting tests · Tamara Hospital

Factor IX assay

Quick answer: This test measures the activity of factor IX, a clotting protein that is low in haemophilia B. It is ordered for unexplained bleeding or a long APTT, to diagnose haemophilia B, and to check carriers.

  • SampleBlood
  • TakesAbout 5 minutes
  • Before the testNo special preparation is usually needed; tell staff about any blood thinners or factor treatment you have had.
  • In fertility careWomen who carry haemophilia may be tested before or during pregnancy to plan a safe delivery.
Infographic

How the test is done

  1. 1Blood is drawn into a citrate tube filled to the line
  2. 2The tube is gently mixed
  3. 3The lab mixes your plasma with plasma that lacks factor IX
  4. 4The clotting time gives your factor IX activity

What your results can mean

Reading your report

Results are given as a percentage of normal activity; discuss them with your doctor.

If the result is outside the usual range

A low level can point to haemophilia B, carrier status, liver disease or vitamin K deficiency. High levels are not usually a concern.

Who usually has this test

People with a long APTT or a bleeding history, and relatives of people with haemophilia B.

Risks and aftercare

Bruising is possible; press firmly on the site if you have a bleeding tendency.

Names and options

Also called: factor 9 assay, FIX, haemophilia B test

Listed by the hospital as

  • FACTOR IX ASSAY, PLASMA

Questions about this test

What is the difference between haemophilia A and B?

Haemophilia A is a lack of factor VIII and haemophilia B is a lack of factor IX. Symptoms are similar, so a factor test tells them apart.

Can newborns be tested for factor IX?

Yes, but newborns naturally have lower factor IX. Results are compared with ranges for their age.

Is haemophilia B inherited?

It is usually passed on through the X chromosome. It mainly affects males, while females are often carriers.

Related tests

Where

Tamara Hospital & IVF Centre, Rajajinagar

34/3, 10th Cross Road, opp. Vardhaka Sardar Patel School, 1st N Block, 2nd Stage, Rajajinagar, Bengaluru 560010

The hospital is open 24 hours. Call before you come to check the timing for your scan or sample collection.

  • Reception, Tamara Hospital RajajinagarReception
  • Waiting area, Tamara Hospital RajajinagarWaiting area
  • IVF lab, Tamara Hospital RajajinagarIVF lab

This page explains each test in general terms so you know what to expect. It is not medical advice: your doctor decides which tests you need and what your results mean. Reference ranges differ between laboratories. Updated 4 November 2026.

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