Anti-dsDNA antibody test
Quick answer: This blood test measures antibodies against double-stranded DNA, which are fairly specific for lupus. Doctors usually order it after a positive ANA when lupus is suspected, and to follow lupus activity, including kidney involvement.
- SampleBlood
- TakesAbout 5 minutes
- Before the testNo special preparation is usually needed.
- In fertility careWomen with lupus are usually advised to plan pregnancy with their doctor when the disease is quiet, and this test helps track activity.
How the test is done
- 1You sit and rest your arm on a support
- 2The nurse cleans a vein and inserts a fine needle
- 3One tube of blood is collected
- 4The lab measures dsDNA antibody levels by immunoassay or immunofluorescence
What your results can mean
Reading your report
Ranges and cut-offs differ between labs and methods, so discuss your result with your doctor.
If the result is outside the usual range
A high level can be seen with lupus, particularly when the disease is active or affecting the kidneys. Levels often fall as lupus settles, and a low or negative result does not rule lupus out.
Who usually has this test
People with a positive ANA and symptoms of lupus, and people with lupus being followed for flares or kidney involvement.
Risks and aftercare
You may have mild soreness or a small bruise for a day or two.
Names and options
Also called: dsDNA, anti double stranded DNA, anti-ds DNA
Listed by the hospital as
- DS DNA
Questions about this test
Can anti-dsDNA levels predict a lupus flare?
In some people, a rising level along with falling complement levels can come before a flare, especially kidney flares. Doctors use the trend together with symptoms and urine tests.
Why is anti-dsDNA checked before pregnancy in lupus?
Pregnancy tends to go better when lupus has been quiet for some months. This test helps your doctor judge how active the disease is before you try to conceive.
Can I have lupus with a negative anti-dsDNA?
Yes. Many people with lupus test negative for anti-dsDNA. Diagnosis is based on your symptoms, examination and a group of tests.
Related tests
Tamara Hospital & IVF Centre, Rajajinagar
34/3, 10th Cross Road, opp. Vardhaka Sardar Patel School, 1st N Block, 2nd Stage, Rajajinagar, Bengaluru 560010
The hospital is open 24 hours. Call before you come to check the timing for your scan or sample collection.
Get directions ↗IVF Pulse fertility care at Tamara Hospital →
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This page explains each test in general terms so you know what to expect. It is not medical advice: your doctor decides which tests you need and what your results mean. Reference ranges differ between laboratories. Updated 4 November 2026.