HCV western blot (immunoblot)
Quick answer: This test looks for antibodies against several hepatitis C virus (HCV) proteins to confirm a positive HCV antibody screening test. It was used to check whether a positive screening result is real; today an HCV RNA (viral load) test is more often used to confirm current infection.
- SampleBlood
- TakesAbout 5 minutes
- Before the testNo special preparation is usually needed.
- In fertility careHepatitis C is part of the infection screening usually done before IVF and in pregnancy.
How the test is done
- 1Blood is drawn from a vein in your arm
- 2Your serum is applied to a strip carrying hepatitis C proteins
- 3Antibodies that bind show up as bands on the strip
- 4The band pattern is read as positive, negative or indeterminate
What your results can mean
Reading your report
Results may be positive, negative or indeterminate, and an HCV RNA test may still be needed; discuss them with your doctor.
If the result is outside the usual range
A positive result confirms antibodies to hepatitis C, meaning past or current infection. An HCV RNA test is needed to show whether the virus is still active.
Who usually has this test
People with a positive hepatitis C antibody screening test.
Risks and aftercare
Bruising is possible; until you know your result, avoid sharing razors or toothbrushes.
Names and options
Also called: hepatitis C confirmatory test, HCV immunoblot, RIBA
Listed by the hospital as
- HCV WESTERNBLOT ASSAY, SERUM
Questions about this test
Does a positive blot mean I have hepatitis C now?
Not always, as antibodies can remain after the infection clears. An HCV RNA test shows whether the virus is still present.
What does an indeterminate result mean?
Some bands are present, but not enough for a clear answer. Doctors may repeat the test or check HCV RNA.
Is hepatitis C curable?
Yes, modern tablet treatments cure most people with hepatitis C. Your doctor will advise on the next steps.
Related tests
Tamara Hospital & IVF Centre, Rajajinagar
34/3, 10th Cross Road, opp. Vardhaka Sardar Patel School, 1st N Block, 2nd Stage, Rajajinagar, Bengaluru 560010
The hospital is open 24 hours. Call before you come to check the timing for your scan or sample collection.
Get directions ↗IVF Pulse fertility care at Tamara Hospital →
Reception
Waiting area
IVF lab
This page explains each test in general terms so you know what to expect. It is not medical advice: your doctor decides which tests you need and what your results mean. Reference ranges differ between laboratories. Updated 4 November 2026.