Anti-HBs (hepatitis B surface antibody) test
Quick answer: This blood test measures antibodies against the hepatitis B surface protein. They show protection from vaccination or past infection. Doctors usually order it to check whether hepatitis B vaccination has worked, for example in health workers, or to decide if you need the vaccine.
- SampleBlood
- TakesAbout 5 minutes
- Before the testNo special preparation is usually needed.
How the test is done
- 1A nurse confirms your details and vaccine history
- 2A vein on your arm is cleaned
- 3A needle collects a tube of blood
- 4The lab measures the level of hepatitis B surface antibody
What your results can mean
Reading your report
Labs report a level in mIU/mL and the protective cut-off they use; discuss with your doctor whether you need a booster.
If the result is outside the usual range
A level above the lab's protective cut-off usually means you are protected, from vaccination or past infection. A low or absent level can mean the vaccine has not worked, protection has faded, or you have never been vaccinated.
Who usually has this test
Health workers, people after a hepatitis B vaccine course, partners of people with hepatitis B, and people on dialysis.
Risks and aftercare
No special aftercare; your doctor may suggest a booster or full vaccine course depending on the level.
Names and options
Also called: anti-HBs, HBsAb, hepatitis B antibody, hepatitis B immunity test
Listed by the hospital as
- ANTI HBS, SERUM
Questions about this test
When should anti-HBs be checked after vaccination?
It is usually checked about 1 to 2 months after the last vaccine dose. Testing much later may show a low level even if you were protected at first.
What if my anti-HBs stays low after vaccination?
Your doctor may suggest another vaccine course and a repeat test. A small number of people do not respond even after two courses.
Is anti-HBs needed before IVF?
It is not always required, but some clinics check it, especially if a partner has hepatitis B. If you are not protected, you may be offered the vaccine.
Related tests
Tamara Hospital & IVF Centre, Rajajinagar
34/3, 10th Cross Road, opp. Vardhaka Sardar Patel School, 1st N Block, 2nd Stage, Rajajinagar, Bengaluru 560010
The hospital is open 24 hours. Call before you come to check the timing for your scan or sample collection.
Get directions ↗IVF Pulse fertility care at Tamara Hospital →
Reception
Waiting area
IVF lab
This page explains each test in general terms so you know what to expect. It is not medical advice: your doctor decides which tests you need and what your results mean. Reference ranges differ between laboratories. Updated 4 November 2026.