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Fertility Tests for Women: What Each One Checks, and When

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Written by IVFPulse Editorial TeamPublished Updated
Fertility Tests for Women: What They Reveal
AI summary

ASRM advises beginning fertility evaluation after 12 months of unprotected intercourse in women under 35, and after 6 months in women aged 35 or older. The core workup assesses ovulation, tubal patency and the uterine cavity; ovarian reserve tests such as AMH and antral follicle count estimate egg quantity, not the chance of conceiving.

  • ASRM advises evaluation at 12 months under 35, and at 6 months from age 35.
  • In women over 40, more immediate evaluation and treatment may be warranted.
  • AMH and antral follicle count are measured on days 2-3 of the cycle and correlate strongly with each other.
  • HSG assesses tubal patency and is usually scheduled 7-10 days after the first day of the period.
  • Ovarian reserve markers estimate egg quantity; they do not predict natural conception.

When should I have fertility tests?

Earlier than most people are told, and the threshold changes with age. ASRM advises beginning evaluation after twelve months of unprotected intercourse in women under 35, and after six months in women aged 35 or older.

Above 40, the guidance shifts again: more immediate evaluation and treatment may be warranted rather than waiting out a fixed window at all.

  • Under 35 — after about 12 months.
  • 35 and over — after about 6 months.
  • Over 40 — sooner, without waiting a set period.
  • Any age — sooner if periods are irregular or absent.

Being investigated is not the same as being treated. Most of the workup exists to find out whether anything needs treating at all.

When to start evaluation12 vs 6 monthsTwelve months of unprotected intercourse under age 35, six months from age 35. Above 40, more immediate evaluation may be warranted.ASRM committee opinion, fertility evaluation of infertile women (2021)

What do AMH and antral follicle count tell you?

How many eggs are likely to remain, and how a stimulation cycle would probably go. They are measured on days two to three of the cycle and correlate strongly with each other, which is why using both adds less than it appears to.

What they do not do is predict whether you will conceive naturally. That distinction is the single most misunderstood part of fertility testing, and it causes a lot of avoidable alarm.

  • AMH — a blood test, stable across the cycle, estimates remaining follicles.
  • Antral follicle count — an ultrasound count of visible small follicles.
  • The two correlate strongly; one is usually enough.
  • Neither describes the quality of the eggs.

Our article on what AMH does and does not measure covers this in detail, and diminished ovarian reserve covers what a low result does and does not mean.

Ovarian reserve tests answer 'how many eggs are left?'. Most people asking for one are asking 'will I get pregnant?' — a question these tests were never designed to answer.

Appointment coming up?

IVY can explain which tests you are likely to be offered, what each one rules in or out, and what to ask.

How are the tubes and uterus checked?

Usually by hysterosalpingography, an X-ray with contrast that shows whether the fallopian tubes are open and outlines the shape of the uterine cavity. It is typically scheduled seven to ten days after the first day of your period.

That timing is deliberate: it sits after bleeding has stopped and before ovulation, which minimises the chance of the test being done in an early pregnancy.

  • HSG — checks tubal patency and the uterine cavity outline.
  • Saline sonography — an alternative for assessing the cavity.
  • Laparoscopy — direct visualisation, but surgery, so not first-line.
  • A proximal blockage on one HSG frequently is not a blockage.

That last point matters enough to repeat: our article on tubal blockages covers why a single abnormal HSG usually warrants repeating rather than acting on.

How is ovulation confirmed?

Usually by a blood progesterone taken in the second half of the cycle, timed to roughly a week before the next period is due. A raised level indicates ovulation occurred in that cycle.

Regular, predictable periods are themselves reasonable evidence that ovulation is happening. That is why irregular or absent periods move testing earlier — they point at the mechanism directly, rather than requiring a year of trying to reveal it.

  • Mid-luteal progesterone — confirms ovulation happened that cycle.
  • Cycle regularity — informative on its own.
  • Ovulation predictor kits — detect the LH surge, not ovulation itself.
  • One anovulatory cycle is not a diagnosis; the pattern matters.

Thyroid function and prolactin are commonly checked alongside, because both can disrupt ovulation and both are straightforward to correct once identified. Our article on TSH levels and fertility covers where the thyroid thresholds actually sit.

What the workup will not tell you

A complete normal workup is common and does not mean nothing is wrong. It means the available tests found nothing, which is a narrower statement than it sounds.

  • No test measures egg quality directly.
  • Unexplained infertility is a real and frequent result.
  • A normal result does not rule out a male factor — test both partners.
  • Reserve testing should not be used to decide whether to try.

The partner's semen analysis is the fastest and cheapest test in the whole workup and is often left until last. Doing it early avoids months of investigating one person for a problem that involves both. Our guide to reading a semen analysis covers what those numbers mean.

Want your results explained?

Upload them and IVY will set out what each test showed, what it rules out, and what usually comes next.

Keep reading

3 Sources

  1. Fertility evaluation of infertile women: a committee opinion (2021). Practice Committee of the American Society for Reproductive Medicine. Fertility and Sterility (2021)
  2. Definition of infertility: a committee opinion (2023). Practice Committee of the American Society for Reproductive Medicine. Fertility and Sterility (2023)
  3. Testing and interpreting measures of ovarian reserve: a committee opinion (2020). Practice Committee of the American Society for Reproductive Medicine. Fertility and Sterility 2020;114:1151–7

Frequently asked questions

What people ask before their first fertility appointment.

When should a woman get fertility tested?

ASRM advises beginning evaluation after 12 months of unprotected intercourse in women under 35, and after 6 months in women aged 35 or older. In women over 40, more immediate evaluation and treatment may be warranted rather than waiting a set period. Testing sooner is reasonable at any age if periods are irregular or absent.

What is the main fertility test for women?

There isn't a single one. The core workup checks three things: whether ovulation is happening, whether the fallopian tubes are open, and whether the uterine cavity is normal. Ovarian reserve markers such as AMH and antral follicle count are usually added, measured on days 2–3 of the cycle, to estimate how many eggs remain.

Does a low AMH mean I cannot get pregnant?

No. AMH estimates how many eggs remain and predicts how a stimulation cycle is likely to go. It is a poor predictor of natural conception, and guideline bodies advise against using a low result alone to deny access to treatment. A low AMH changes how a cycle is planned rather than whether conception is possible.

What happens if all my fertility tests are normal?

That is a common outcome and is called unexplained infertility. It means the available tests found nothing, not that nothing is wrong — no test measures egg quality directly, for instance. Treatment can still be offered on the basis of age and how long you have been trying, without a specific diagnosis.